Blended Mobility Network - Technical innovations in basics and translational research: Applications to Immunology-Oncology

Second edition of the mixed mobility program in immunology-oncology of the Institute Cancer and Immunology within CIVIS, the alliance of European civic universities.

"Technical innovations in basic and translational research in Immunology-Oncology

6 ECTS (Masters) - Training hours (PhD students)

Application from 1st to 30th November 2022 (link below)
https://civis.eu/en/civis-courses/technical-innovations-applications-to-immuno-oncology

Program

The fundamental objective of the proposed program is to prepare future scientists and physicians for the major evolutions of current concepts in the field of immuno-oncology by approaching the issues from a technical perspective. Thus, the teaching will focus on technical applications in basic, translational and clinical immuno-oncology, through a mixed mobility curriculum. The virtual part consists of 5 courses (cytometry, organoids, genomics, proteomics and imaging-microscopy). The physical part consists of a one-week mobility in high-tech laboratories and scientific platforms at Aix-Marseille University.

The courses of the virtual part contain digital capsules of about 60 minutes each as follows Students will receive the theoretical and technical basis to understand the functioning of these innovative technologies, as well as the applications to the exploration of anti-tumor immunity in aspects ranging from basic research to clinical approaches. The critical step of data analysis using machine learning algorithms will also be addressed in the different themes.

1. Cytometry (mass, flow and spectral):
   • Multi-color flow cytometry for phenotypic characterization of tumor-infiltrating T cells, Silvia Piconese, Sapienza Università di Roma
   • Real-time cell analysis in immuno-oncology, Mihaela Economescu, Stefan S. Nicolau Institute of Virology, Bucharest Romania
   • Multiparametric flow cytometry analysis for phenotypic characterization of age-associated changes in circulating Tcell subsets, Maria Mittelbrunn, Universidad Autonoma de Madrid
   • Evaluation of neutrophil functions in the tumor microenvironment, Luminita Marutescu, University of Bucharest
   • Cancer immunomonitoring - NK cells and cancer, Gratiela Gradisteanu, University of Bucharest
   • Detection of immune checkpoints and their ligands in the tumor environment by multicolor flow cytometry, Lilia Matei, Stefan S. Nicolau Institute of Virology, Bucharest, Romania
   • Evaluation of immune function in tumors using xMAP multiplex technology, Coralia Bleotu, Stefan S. Nicolau Institute of Virology, Bucharest Romania
   • Multicolor imaging flow cytometry for characterization of cancer response to drug treatments, Cristina Elena Staicu, National Institute of Laser, Plasma and Radiation Physics, University of Bucharest; Livia Elena Sima, Institute of Biochemistry of the Romanian Academy
   • Mass Cytometry - Principles and Applications, Amira A Ben Amara, Aix-Marseille University
   • Spectral flow cytometry, Manon Richaud, Institut Paoli Calmettes, CRCM, Aix-Marseille University
   • Computational methods for flow and mass cytometry data analysis, Anne-Sophie Chretien, Aix-Marseille University.
2. Organoids :
   • Organoids: New biological models for developmental biology and cancer research, Géraldine Guasch, CRCM, Aix-Marseille University
   • Ex vivo culture of organoids for personalized medicine and regenerative medicine, Marie Isabelle Garcia, Université Libre de Bruxelles
   • 3D in vitro models of the human nervous system: challenges and opportunities, Alessandro Rosa, Sapienza Università di Roma
   • In vitro evaluation of cellular energy metabolism and cell growth, Petronila Penela; Angela Albitre; Laura Formentini, Universidad Autónoma de Madrid
   • Animal models for tumor immunology/mouse models for cancer immunotherapy research, Catalina Ribas; Kostas Stamatakis; María Sanz, Universidad Autónoma de Madrid
3. Genomics (CRSPR, RNAseq and TCRseq):
   • Functional genomics using CRISPR screening, Sandrine Roulland, Aix-Marseille University
   • DNA microarrays and RNAseq in cancer transcriptomics, Rodolfo Negri, Sapienza University of Rome
   • Single cell RNA-seq (scRNAseq), Pierre Milpied, CIML, Aix-Marseille University
   • TCR/CDR3 sequencing: insights into T cell development and anti-cancer response, David Vermijlen, Center for Research in Immunology (U-CRI), Université Libre de Bruxelles
   • Hands-on Introduction to RNA-Seq, Sumeet Pal Singh, IRIBHM, Université Libre de Bruxelles
4. Proteomics:
   • Protein analysis by mass spectrometry, Marco Crescenzi, Sapienza Università di Roma, Istituto Superiore di Sanità; Serena Camerini, Istituto Superiore di Sanità
   • Characterization of IgG glycosylation and its impact in cancer and immunology, Cédric Delporte; Yosra Helali, Université Libre de Bruxelles
   • Reverse phase protein arrays: Identification of protein biomarkers of diseases, José M. Cuezva, Universidad Autónoma de Madrid
   • Targeted Proteomics: Development of quantitative proteomics based on parallel reaction monitoring (PRM) applied to her2-positive breast cancer, Luc Camoin, Aix-Marseille University
   • Maldi imaging and therapeutic antibodies: application to glioma, Daniel Lafitte, Aix-Marseille University
5. Microscopy-Imaging
   • Cryo-electron microscopy (Cryo-EM) for the study of fine cellular structures and protein complexes in immune cells and cancer cells, Luciana Dini, Sapienza Università di Roma
   • 3D structured illumination microscopy for the study of nuclear integrity and cancer invasion, Isabella Saggio, Sapienza Universita di Roma
   • Spatio-temporal organization of macromolecular complexes involved in lymphocyte signaling, Didier Marguet, CIML, Aix-Marseille University
   • Isolation and characterization techniques for the study of exosomes, María Yáñez-Mó, Universidad Autónoma de Madrid; Mar Valés-Gómez; Noa Martín Cofreces

The host laboratories for the physics part are listed below. This part will allow students to put into practice what they have learned in the virtual part.

• Luc Camoin (Platform Proteomics, CRCM)
• Alice Carrier, (Laboratory Juan Iovanna, CRCM)
• Patrick Chames (laboratory of Patrick Chames, CRCM)
• Emmanuelle Charafe (Platform IPC/CRCM Experimental Pathology, CRCM)
• Anne-Sophie Chrétien (Laboratory of Daniel Olive, CRCM)
• Raynier Devillier (Laboratory of Daniel Olive, CRCM)
• Mathilde Poplineau (Laboratory of Estelle Duprez, CRCM)
• Géraldine Guasch (Platform 3D-Hub-Organoids, CRCM)
• Paula Michea (Laboratory of Jean-Paul Borg, CRCM)
• Geoffrey Guittard (Laboratory of Jacques Nunes, CRCM)
• Erinn Soucie, (Laboratory of Juan Iovanna, CRCM)
• Richard Tomassini (Laboratory of Juan Iovanna, CRCM)
• Sophie Vasseur (Laboratory of Juan Iovanna, CRCM)
• Sandrine Henri (Laboratory of Bernard Malissen, CIML)
• Didier Marguet (Plateform ImagImm, CIML)
• Pierre Milpied (Laboratory of Pierre Milpied, CIML)
• Sandrine Roulland (Laboratory of Sandrine Roulland, CIML)
• Flavio Maina (Laboratory of Flavio Maina, IBDM)

Registration

Closing date for registration is 30.11.2022
Selection of candidates, deadline 07.12.2022

Organizing Committee

• Paula Michea, Aix-Marseille University (AMU), Institute of Cancer and Immunology (ICI), Marseille Faculty of Sciences, Marseille Cancer Research Center (CRCM)
• Marie-dominique Franco, Project Manager, Institute of Cancer and Immunology (ICI, AMU)
• Luminita Marutescu, University of Bucharest, Faculty of Biology, Department of Biotechnology and Renewable Resources, Systems Biology and Ecology Research Platform
• Catalina Ribas and María Yánez Mo, Autonomous University of Madrid, Faculty of Science, Department of Molecular Biology.
• Loretta Tuosto, Sapienza Università di Roma, Faculty of Mathematical, Physical and Natural Sciences, Charles Darwin Biology and Biotechnology Department (BBCD)
• David Vermijlen, ULB Center for Research in Immunology (U-CRI), Faculty of Pharmacy, Department of Pharmacotherapy and Pharmacy and Center for Research in Immunology

About CIVIS

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